Fiche publication
Date publication
mars 2022
Journal
Proceedings of the National Academy of Sciences of the United States of America
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
,
Dr MARCHAND Virginie
Tous les auteurs :
Khoshnevis S, Dreggors-Walker RE, Marchand V, Motorin Y, Ghalei H
Lien Pubmed
Résumé
SignificanceThe presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2'--methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2'--methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2'--methylations to ribosome activities is unknown. By establishing a method to disturb rRNA 2'--methylation patterns, we show that rRNA 2'--methylations affect the function and fidelity of the ribosome and change the balance between different ribosome conformational states. Our work links 2'--methylation to ribosome dynamics and defines a set of critical rRNA 2'--methylations required for ribosome biogenesis and others that are dispensable.
Mots clés
2′-O-methylation, rRNA modification, ribosome biogenesis, ribosome dynamics, translation regulation
Référence
Proc Natl Acad Sci U S A. 2022 Mar 22;119(12):e2117334119
