Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
,
Dr MARCHAND Virginie
Tous les auteurs :
Marchand V, Ayadi L, Bourguignon-Igel V, Motorin Y
Lien Pubmed
Résumé
Recent advances in high-throughput sequencing have shed new light on the diversity of small noncoding RNA (sncRNA) classes and their crucial roles in gene regulation and disease. One key step in sncRNA profiling consists in their quantification and assessment of their degradation extent. In this chapter, we will describe different gold standard methods used to achieve both purposes before using the sncRNAs in downstream applications.
Mots clés
Capillary electrophoresis, Fluorescence, RNA integrity number, RNA quantification, UV spectroscopy
Référence
Methods Mol Biol. 2021 ;2300:17-29
