Lin J, Agote-Aran A, Liao Y, Cloarec M, Andronov L, Schoch RL, Ronchi P, Cochard V, Zhu R, Grandgirard E, Liu X, Lemée MV, Kleiss C, Golzio C, Ruff M, Chevreux G, Schwab Y, Klaholz BP, Sumara I

Nat Commun. 2026 03 25;:

Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus.

Kohl MP, Bahena-Ceron R, Chane-Woon-Ming B, Kompatscher M, Erlacher MD, Barchet C, von Loeffelholz O, Romby P, Klaholz BP, Marzi S

Nat Commun. 2026 02 12;:

Explicit correction of severely non-uniform distributions of cryo-EM views.

Barchet C, von Loeffelholz O, Bahena-Ceron R, Klaholz BP, Urzhumtsev AG

Acta Crystallogr D Struct Biol. 2026 02 1;:

Structural Insights into Ciona intestinalis Septins: complexes suggest a mechanism for nucleotide-dependent interfacial cross-talk.

Mendonça DC, Morais STB, Ciol H, Pinto APA, Leonardo DA, D'Muniz Pereira H, Valadares NF, Portugal RV, Klaholz BP, Garratt RC, Araujo APU

J Mol Biol. 2024 07 1;:168693

UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope.

Liao Y, Andronov L, Liu X, Lin J, Guerber L, Lu L, Agote-Arán A, Pangou E, Ran L, Kleiss C, Qu M, Schmucker S, Cirillo L, Zhang Z, Riveline D, Gotta M, Klaholz BP, Sumara I

J Cell Biol. 2024 07 1;223(7):

The structure of the human 80S ribosome at 1.9 Å resolution reveals the molecular role of chemical modifications and ions in RNA.

Holvec S, Barchet C, Lechner A, Fréchin L, De Silva SNT, Hazemann I, Wolff P, von Loeffelholz O, Klaholz BP

Nat Struct Mol Biol. 2024 06 6;:

Program : analysing distributions of cryo-EM projections using uniform spherical grids.

Urzhumtseva L, Barchet C, Klaholz BP, Urzhumtsev AG

J Appl Crystallogr. 2024 06 1;57(Pt 3):865-876

RlmQ: A Newly Discovered rRNA Modification Enzyme Bridging RNA Modification and Virulence Traits in .

Bahena-Ceron R, Teixeira C, Jaramillo Ponce JR, Wolff P, Couzon F, François P, Klaholz B, Vandenesch F, Romby P, Moreau K, Marzi S

RNA. 2023 12 19;:

MODOMICS: a database of RNA modifications and related information. 2023 update.

Cappannini A, Ray A, Purta E, Mukherjee S, Boccaletto P, Moafinejad SN, Lechner A, Barchet C, Klaholz BP, Stefaniak F, Bujnicki JM

Nucleic Acids Res. 2023 11 28;:

Focused classifications and refinements in high-resolution single particle cryo-EM analysis.

Barchet C, Fréchin L, Holvec S, Hazemann I, von Loeffelholz O, Klaholz BP

J Struct Biol. 2023 08 31;:108015

Asymmetric dimerization in a transcription factor superfamily is promoted by allosteric interactions with DNA.

Patel AKM, Vilela P, Shaik TB, McEwen AG, Hazemann I, Brillet K, Ennifar E, Hamiche A, Markov GV, Laudet V, Moras D, Klaholz BP, Billas IML

Nucleic Acids Res. 2023 07 28;:

Quaternary glucocorticoid receptor structure highlights allosteric interdomain communication.

Postel S, Wissler L, Johansson CA, Gunnarsson A, Gordon E, Collins B, Castaldo M, Köhler C, Öling D, Johansson P, Fröderberg Roth L, Beinsteiner B, Dainty I, Delaney S, Klaholz BP, Billas IML, Edman K

Nat Struct Mol Biol. 2023 02 6;:

MDSPACE: Extracting continuous conformational landscapes from cryo-EM single particle datasets using 3D-to-2D flexible fitting based on Molecular Dynamics simulation.

Vuillemot R, Mirzaei A, Harastani M, Hamitouche I, Fréchin L, Klaholz BP, Miyashita O, Tama F, Rouiller I, Jonic S

J Mol Biol. 2023 01 10;:167951

Nucleosome dyad determines the H1 C-terminus collapse on distinct DNA arms.

Louro JA, Boopathi R, Beinsteiner B, Mohideen Patel AK, Cheng TC, Angelov D, Hamiche A, Bendar J, Kale S, Klaholz BP, Dimitrov S

Structure. 2022 12 26;:

splitSMLM, a spectral demixing method for high-precision multi-color localization microscopy applied to nuclear pore complexes.

Andronov L, Genthial R, Hentsch D, Klaholz BP

Commun Biol. 2022 10 17;5(1):1100

High-resolution cryo-EM performance comparison of two latest-generation cryo electron microscopes on the human ribosome.

Fréchin L, Holvec S, von Loeffelholz O, Hazemann I, Klaholz BP

J Struct Biol. 2022 10 11;:107905

Structural basis for safe and efficient energy conversion in a respiratory supercomplex.

Kao WC, Ortmann de Percin Northumberland C, Cheng TC, Ortiz J, Durand A, von Loeffelholz O, Schilling O, Biniossek ML, Klaholz BP, Hunte C

Nat Commun. 2022 Jan 27;13(1):545

Studying the Structural Organization of Polyribosomes with Alexander S. Spirin.

Klaholz BP

Biochemistry (Mosc). 2021 Sep;86(9):1053-1059

MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.

Ibrahim A, Papin C, Mohideen-Abdul K, Le Gras S, Stoll I, Bronner C, Dimitrov S, Klaholz BP, Hamiche A

Science. 2021 Jun 25;372(6549):

Myod1 and GR coordinate myofiber-specific transcriptional enhancers.

Rovito D, Rerra AI, Ueberschlag-Pitiot V, Joshi S, Karasu N, Dacleu-Siewe V, Rayana KB, Ghaibour K, Parisotto M, Ferry A, Jelinsky SA, Laverny G, Klaholz BP, Sexton T, Billas IML, Duteil D, Metzger D

Nucleic Acids Res. 2021 Apr 9;:

Identification of Mg ions next to nucleotides in cryo-EM maps using electrostatic potential maps.

Wang J, Natchiar SK, Moore PB, Klaholz BP

Acta Crystallogr D Struct Biol. 2021 Apr 1;77(Pt 4):534-539

Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORM.

Andronov L, Vonesch JL, Klaholz BP

Methods Mol Biol. 2021 ;2247:271-286

Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection.

von Loeffelholz O, Klaholz BP

Methods Mol Biol. 2021 ;2305:291-299

Ribosomal RNA 2'O-methylation as a novel layer of inter-tumour heterogeneity in breast cancer.

Marcel V, Kielbassa J, Marchand V, Natchiar KS, Paraqindes H, Nguyen Van Long F, Ayadi L, Bourguignon-Igel V, Lo Monaco P, Monchiet D, Scott V, Tonon L, Bray SE, Diot A, Jordan LB, Thompson AM, Bourdon JC, Dubois T, André F, Catez F, Puisieux A, Motorin Y, Klaholz BP, Viari A, Diaz JJ

NAR Cancer. 2020 Dec;2(4):zcaa036

Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss.

Orlov I, Hemmer C, Ackerer L, Lorber B, Ghannam A, Poignavent V, Hleibieh K, Sauter C, Schmitt-Keichinger C, Belval L, Hily JM, Marmonier A, Komar V, Gersch S, Schellenberger P, Bron P, Vigne E, Muyldermans S, Lemaire O, Demangeat G, Ritzenthaler C, Klaholz BP

Proc. Natl. Acad. Sci. U.S.A.. 2020 May 5;:

Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes.

Bhaskar V, Graff-Meyer A, Schenk AD, Cavadini S, von Loeffelholz O, Natchiar SK, Artus-Revel CG, Hotz HR, Bretones G, Klaholz BP, Chao JA

Cell Rep. 2020 Apr 7;31(1):107473

Targeting the Human 80S Ribosome in Cancer: From Structure to Function and Drug Design for Innovative Adjuvant Therapeutic Strategies.

Gilles A, Frechin L, Natchiar K, Biondani G, Loeffelholz OV, Holvec S, Malaval JL, Winum JY, Klaholz BP, Peyron JF

Cells. 2020 Mar 5;9(3):

Deriving and refining atomic models in crystallography and cryo-EM: the latest Phenix tools to facilitate structure analysis.

Klaholz BP

Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):878-881

CENP-A nucleosome clusters form rosette-like structures around HJURP during G1.

Andronov L, Ouararhni K, Stoll I, Klaholz BP, Hamiche A

Nat Commun. 2019 Sep 30;10(1):4436

NMethyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation.

Ma H, Wang X, Cai J, Dai Q, Natchiar SK, Lv R, Chen K, Lu Z, Chen H, Shi YG, Lan F, Fan J, Klaholz BP, Pan T, Shi Y, He C

Nat. Chem. Biol.. 2019 01;15(1):88-94

Tetracyclines Modify Translation by Targeting Key Human rRNA Substructures.

Mortison JD, Schenone M, Myers JA, Zhang Z, Chen L, Ciarlo C, Comer E, Natchiar SK, Carr SA, Klaholz BP, Myers AG

Cell Chem Biol. 2018 Dec 20;25(12):1506-1518.e13

Visualizing the Role of 2'-OH rRNA Methylations in the Human Ribosome Structure.

Natchiar SK, Myasnikov AG, Hazemann I, Klaholz BP

Biomolecules. 2018 10 25;8(4):

New tools for the analysis and validation of cryo-EM maps and atomic models.

Afonine PV, Klaholz BP, Moriarty NW, Poon BK, Sobolev OV, Terwilliger TC, Adams PD, Urzhumtsev A

Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):814-840

Volta phase plate data collection facilitates image processing and cryo-EM structure determination.

von Loeffelholz O, Papai G, Danev R, Myasnikov AG, Natchiar SK, Hazemann I, Ménétret JF, Klaholz BP

J. Struct. Biol.. 2018 Jun;202(3):191-199

Structure-function analyses unravel distinct effects of allosteric inhibitors of HIV-1 integrase on viral maturation and integration.

Bonnard D, Le Rouzic E, Eiler S, Amadori C, Orlov I, Bruneau JM, Brias J, Barbion J, Chevreuil F, Spehner D, Chasset S, Ledoussal B, Moreau F, Saïb A, Klaholz BP, Emiliani S, Ruff M, Zamborlini A, Benarous R

J. Biol. Chem.. 2018 Apr 20;293(16):6172-6186

3DClusterViSu: 3D clustering analysis of super-resolution microscopy data by 3D Voronoi tessellations.

Andronov L, Michalon J, Ouararhni K, Orlov I, Hamiche A, Vonesch JL, Klaholz BP

Bioinformatics. 2018 Apr 4;:

Structural features of the salivary gland hypertrophy virus of the tsetse fly revealed by cryo-electron microscopy and tomography.

Orlov I, Drillien R, Spehner D, Bergoin M, Abd-Alla AMM, Klaholz BP

Virology. 2018 01 15;514:165-169

Visualization of chemical modifications in the human 80S ribosome structure.

Natchiar SK, Myasnikov AG, Kratzat H, Hazemann I, Klaholz BP

Nature. 2017 11 23;551(7681):472-477

Focused classification and refinement in high-resolution cryo-EM structural analysis of ribosome complexes.

von Loeffelholz O, Natchiar SK, Djabeur N, Myasnikov AG, Kratzat H, Ménétret JF, Hazemann I, Klaholz BP

Curr. Opin. Struct. Biol.. 2017 10;46:140-148

The Ribosome Holds the RNA Polymerase on Track in Bacteria.

Klaholz BP

Trends Biochem. Sci.. 2017 09;42(9):686-689

The integrative role of cryo electron microscopy in molecular and cellular structural biology.

Orlov I, Myasnikov AG, Andronov L, Natchiar SK, Khatter H, Beinsteiner B, Ménétret JF, Hazemann I, Mohideen K, Tazibt K, Tabaroni R, Kratzat H, Djabeur N, Bruxelles T, Raivoniaina F, Pompeo LD, Torchy M, Billas I, Urzhumtsev A, Klaholz BP

Biol. Cell. 2017 Feb;109(2):81-93

Importance of the Sequence-Directed DNA Shape for Specific Binding Site Recognition by the Estrogen-Related Receptor.

Mohideen-Abdul K, Tazibt K, Bourguet M, Hazemann I, Lebars I, Takacs M, Cianférani S, Klaholz BP, Moras D, Billas IML

Front Endocrinol (Lausanne). 2017 ;8:140

Cryo-EM study of start codon selection during archaeal translation initiation.

Coureux PD, Lazennec-Schurdevin C, Monestier A, Larquet E, Cladière L, Klaholz BP, Schmitt E, Mechulam Y

Nat Commun. 2016 11 7;7:13366

Structure-function insights reveal the human ribosome as a cancer target for antibiotics.

Myasnikov AG, Kundhavai Natchiar S, Nebout M, Hazemann I, Imbert V, Khatter H, Peyron JF, Klaholz BP

Nat Commun. 2016 Sep 26;7:12856

Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation.

Martin F, Ménétret JF, Simonetti A, Myasnikov AG, Vicens Q, Prongidi-Fix L, Natchiar SK, Klaholz BP, Eriani G

Nat Commun. 2016 08 24;7:12622

SharpViSu: integrated analysis and segmentation of super-resolution microscopy data.

Andronov L, Lutz Y, Vonesch JL, Klaholz BP

Bioinformatics. 2016 Jul;32(14):2239-41

ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy.

Andronov L, Orlov I, Lutz Y, Vonesch JL, Klaholz BP

Sci Rep. 2016 Apr;6:24084

IBiSS, a versatile and interactive tool for integrated sequence and 3D structure analysis of large macromolecular complexes.

Beinsteiner B, Michalon J, Klaholz BP

Bioinformatics. 2015 Oct 15;31(20):3339-44

Structure-function relationships in nuclear receptors: the facts.

Moras D, Billas IM, Rochel N, Klaholz BP

Trends Biochem Sci. 2015 Jun;40(6):287-90

X-ray and Cryo-electron Microscopy Structures of Monalysin Pore-forming Toxin Reveal Multimerization of the Pro-form.

Leone P, Bebeacua C, Opota O, Kellenberger C, Klaholz B, Orlov I, Cambillau C, Lemaitre B, Roussel A

J. Biol. Chem.. 2015 May 22;290(21):13191-201

Structure of the human 80S ribosome.

Khatter H, Myasnikov AG, Natchiar SK, Klaholz BP

Nature. 2015 Apr 30;520(7549):640-5

Structural insight into the mechanism of stabilization of the 7SK small nuclear RNA by LARP7.

Uchikawa E, Natchiar KS, Han X, Proux F, Roblin P, Zhang E, Durand A, Klaholz BP, Dock-Bregeon AC

Nucleic Acids Res.. 2015 Mar 31;43(6):3373-88

Conformation transitions of eukaryotic polyribosomes during multi-round translation.

Afonina ZA, Myasnikov AG, Shirokov VA, Klaholz BP, Spirin AS

Nucleic Acids Res.. 2015 Jan;43(1):618-28

Investigating the cellular distribution and interactions of HIV-1 nucleocapsid protein by quantitative fluorescence microscopy.

Anton H, Taha N, Boutant E, Richert L, Khatter H, Klaholz B, Ronde P, Real E, de Rocquigny H, Mely Y

PLoS One. 2015 Feb 27;10(2):e0116921

The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes.

Myasnikov AG, Afonina ZA, Ménétret JF, Shirokov VA, Spirin AS, Klaholz BP

Nat Commun. 2014 Nov 7;5:5294

Cryo-electron microscopy structure of lactococcal siphophage 1358 virion.

Spinelli S, Bebeacua C, Orlov I, Tremblay D, Klaholz BP, Moineau S, Cambillau C

J. Virol.. 2014 Aug;88(16):8900-10

Formation of circular polyribosomes on eukaryotic mRNA without cap-structure and poly(A)-tail: a cryo electron tomography study.

Afonina ZA, Myasnikov AG, Shirokov VA, Klaholz BP, Spirin AS

Nucleic Acids Res.. 2014 Aug;42(14):9461-9

Purification, characterization and crystallization of the human 80S ribosome.

Khatter H, Myasnikov AG, Mastio L, Billas IM, Birck C, Stella S, Klaholz BP

Nucleic Acids Res. 2014 Apr;42(6):e49

The palindromic DNA-bound USP/EcR nuclear receptor adopts an asymmetric organization with allosteric domain positioning.

Maletta M, Orlov I, Roblin P, Beck Y, Moras D, Billas IM, Klaholz BP

Nat Commun. 2014 Jun 19;5:4139

Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor.

Simonetti A, Marzi S, Billas IM, Tsai A, Fabbretti A, Myasnikov AG, Roblin P, Vaiana AC, Hazemann I, Eiler D, Steitz TA, Puglisi JD, Gualerzi CO, Klaholz BP

Proc. Natl. Acad. Sci. U.S.A.. 2013 Sep 24;110(39):15656-61

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