Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS.
Fiche publication
Date publication
janvier 2023
Journal
STAR protocols
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GHIRINGHELLI François, Dr LADOIRE Sylvain, Dr LIMAGNE Emeric
Tous les auteurs :
Kalfeist L, Petit S, Galland L, Ghiringhelli F, Ladoire S, Limagne E
Lien Pubmed
Résumé
The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45 tumor-infiltrating lymphocytes, cancer-associated fibroblasts, and tumor cells. The protocol includes four steps: generation of 4T1 tumors, tumor collection and digestion, magnetic sorting of the different populations, and phenotypic validation of sorted cells. For complete details on the use and execution of this protocol, please refer to Limagne et al. (2022)..
Mots clés
Cancer, Cell isolation, Flow Cytometry/Mass Cytometry, Immunology, Model Organisms
Référence
STAR Protoc. 2023 01 18;4(1):101951