Minimal residual disease detection by multicolor flow cytometry in cryopreserved ovarian tissue from leukemia patients.

Fiche publication


Date publication

janvier 2022

Journal

Journal of ovarian research

Auteurs

Membres identifiés du Cancéropôle Est :
Dr AMIOT Clotilde, Pr GARNACHE-OTTOU Francine, Dr KOSCINSKI Isabelle, Pr ROUX Christophe


Tous les auteurs :
Zver T, Frontczak S, Poirot C, Rives-Feraille A, Leroy-Martin B, Koscinski I, Arbez-Gindre F, Garnache-Ottou F, Roux C, Amiot C

Résumé

Cryopreservation of ovarian tissue is a fertility-preservation option for women before gonadotoxic treatments. However, cryopreserved ovarian tissue transplantation must be performed with caution in women with malignancies that may metastasize to the ovaries. For this purpose, detecting minimal residual disease (MRD) in the ovarian cortex using sensitive methods is a crucial step. We developed an automated ovarian tissue dissociation method to obtain ovarian cell suspensions.

Mots clés

Fertility preservation, Minimal residual disease, Multicolor flow cytometry, Ovarian tissue cryopreservation

Référence

J Ovarian Res. 2022 Jan 18;15(1):9