The detection of specific hypermethylated WIF1 and NPY genes in circulating DNA by crystal digital PCR™ is a powerful new tool for colorectal cancer diagnosis and screening.
Fiche publication
Date publication
octobre 2021
Journal
BMC cancer
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PRETET Jean-Luc, Dr HERVOUET Eric, Dr SELMANI Zohair
Tous les auteurs :
Overs A, Flammang M, Hervouet E, Bermont L, Pretet JL, Christophe B, Selmani Z
Lien Pubmed
Résumé
In oncology, liquid biopsy is of major relevance from theranostic point of view. The searching for mutations in circulating tumor DNA (ctDNA) in case of colorectal cancers (CRCs) allows the optimization of patient care. In this context, independent of mutation status biomarkers are required for its detection to confirm the presence of ctDNA in liquid biopsies. Indeed, the hypermethylation of NPY and WIF1 genes appear to be an ideal biomarker for the specific detection of ctDNA in CRCs. The objective of this work is to develop the research of hypermethylation of NPY and WIF1 by Crystal Digital PCR™ for the detection of ctDNA in CRCs.
Mots clés
Colorectal cancer, DNA methylation, Digital PCR, Epigenetics, Liquid biopsy, NPY, WIF1
Référence
BMC Cancer. 2021 Oct 10;21(1):1092