Steroidal glycosides from Ornithogalum dubium Houtt.
Fiche publication
Date publication
février 2019
Journal
Phytochemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LIRUSSI Frédéric
Tous les auteurs :
Andriamisaina N, Mitaine-Offer AC, Miyamoto T, Tanaka C, Paululat T, Lirussi F, Lacaille-Dubois MA
Lien Pubmed
Résumé
The phytochemical study of Ornithogalum dubium Houtt. (Asparagaceae) led to the isolation of five undescribed steroidal glycosides together with two known ones. Their structures were established by using NMR analysis and mass spectrometry as (25R)-3β-hydroxyspirost-5-en-1β-yl O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside, (25S)-3β-hydroxyspirost-5-en-1β-yl O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside, (22S)-16β-[(α-L-rhamnopyranosyl)oxy]-22-hydroxycholest-5-en-3β-yl O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside, (22S,23S)-1β,3β,11α,16β,23-pentahydroxy-5α-cholest-24-en-22β-yl β-D-glucopyranoside, (22S,23S)-3β-[(β-D-glucopyranosyl)oxy]-22,23-dihydroxy-5α-cholest-24-en-16β-yl O-α-L-rhamnopyranosyl)-(1 → 4)-β-D-glucopyranoside. Their cytotoxic activities against two human cells, a lung carcinoma A-549 and a promyelocytic leukemia HL-60 cell lines, were evaluated by using the XTT method. The results showed no significant cytotoxicity on the tested cells. The influence of the potentiation of cisplatin cytotoxicity in A-549 cells was also investigated and a slight effect was observed only for the (25R) spirostane-type derivative.
Mots clés
Asparagaceae, Cholestane glycosides, Cytotoxicity, NMR, Ornithogalum dubium, Spirostane glycosides
Référence
Phytochemistry. 2019 Feb 8;160:78-84