Protocol for high-density peptide and protein presentation on biomaterial surfaces using virus-like particles.

Fiche publication

Date publication

juillet 2026

Journal

STAR protocols

Auteurs

Membres identifiés du Cancéropôle Est :
Dr PIEUCHOT Laurent

Tous les auteurs :
Hedna R, Maayouf H, Dos Santos T, Pieuchot L

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/42490226

Résumé

Here, we present a protocol for achieving high-density peptide and protein presentation on biomaterial surfaces using self-assembling virus-like particles. We describe steps for the design, recombinant production, purification, and quality control of peptide- or protein-displaying particles. We also detail procedures for their use in functionalizing polydimethylsiloxane (PDMS) and other solid substrates. The approach enables multivalent and spatially controlled ligand presentation and is applicable to reproducible cell-material interaction studies and the biofunctionalization of implant-relevant surfaces. For complete details on the use and execution of this protocol, please refer to Maayouf et al..