AMITO: An open-access application for assisted analysis of mitochondria in transmission electron microscopy images.

Fiche publication

Date publication

août 2026

Journal

American journal of physiology. Cell physiology

Auteurs

Membres identifiés du Cancéropôle Est :
Dr HUCTEAU Elyse , Dr MALLARD Joris

Tous les auteurs :
Hucteau E, Hureau TJ, Pagano AF, Mallard J

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/42612150

Résumé

Analysis of mitochondrial morphology and ultrastructure from transmission electron microscopy (TEM) images is essential for understanding cellular adaptations in both physiological and pathological conditions. However, these investigations remain time-consuming, poorly standardized and therefore highly variable. We developed an open-access, standalone MATLAB-based application (AMITO) that can be run without license and integrates analyses within a single user-friendly interface. AMITO demonstrated excellent agreement with conventional manual image analysis software, high intra- and inter-experimenter reproducibility, and substantial reduction in analysis time. Therefore, AMITO provides a reliable, efficient, and accessible tool to ensure robustness and reproducibility in mitochondrial assessment.