Fiche publication
Date publication
juin 2026
Journal
Nature plants
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEINLEIN Manfred
Tous les auteurs :
Sede AR, Moorlach B, Galli M, Ladera Carmona MJ, Patel A, Heinlein M, Kogel KH
Lien Pubmed
Résumé
Gene silencing mediated by double-stranded RNA (dsRNA) is a promising strategy for crop protection. However, unlike applications targeting fungi or insects, effective antiviral use of exogenous dsRNA requires delivery into the plant cytoplasm, where viruses replicate. Limited cellular uptake and rapid instability on leaf surfaces have driven the development of diverse nanocarrier platforms, including layered double hydroxides, carbon nanotubes, mesoporous silica nanoparticles, chitosan complexes, lipid-based particles and bacterially encapsulated dsRNA. Although these systems often enhance dsRNA stability and apoplastic accumulation, robust symplastic delivery remains poorly characterized. Here we critically examine the gap between experimental success and agronomic performance of nanocarrier-enabled dsRNA delivery systems. Comparative analyses suggest that carrier dose, size, ζ-potential and organic phase affinity influence systemic translocation within the plant, providing a framework for rational carrier design. Defining such principles will be essential for translating RNA-based antiviral strategies into reliable field applications.
Référence
Nat Plants. 2026 06 11;:
