Fiche publication
Date publication
mai 2026
Journal
Journal of microscopy
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAEBERLE Olivier
Tous les auteurs :
Pestana Legori L, Chacón Ávila CA, Debailleul M, Verrier N, Haeberlé O
Lien Pubmed
Résumé
Tomographic Diffractive Microscopy (TDM) provides label-free three-dimensional imaging of transparent samples with resolution surpassing confocal limits. At IRIMAS, successive instrumental developments since 2009 have enhanced TDM capabilities through transmission, reflection, isotropic, polarisation-sensitive, and dual-view configurations. However, conventional single-wavelength TDM cannot distinguish chemically distinct structures with similar optical properties at the measurement wavelength. This article presents the development of hyperspectral TDM, which exploits wavelength-dependent optical properties to provide chemical specificity through 3D spectroscopic characterisation. We describe the physical principles underlying TDM reconstruction, detail our integration of a supercontinuum source for multi-wavelength capability, and outline applications in biomedical and material imaging. Our structured development roadmap encompasses instrumentation, calibration, hyperspectral synthesis, and AI-enhanced reconstruction.
Mots clés
hyperspectral imaging, label‐free microscopy, quantitative phase imaging, tomographic diffractive microscopy
Référence
J Microsc. 2026 05 6;:
