Fiche publication
Date publication
juin 2018
Journal
Frontiers in plant science
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GAQUEREL Emmanuel
Tous les auteurs :
Schuman MC, Meldau S, Gaquerel E, Diezel C, McGale E, Greenfield S, Baldwin IT
Lien Pubmed
Résumé
The jasmonate hormones are essential regulators of plant defense against herbivores and include several dozen derivatives of the oxylipin jasmonic acid (JA). Among these, the conjugate jasmonoyl isoleucine (JA-Ile) has been shown to interact directly with the jasmonate co-receptor complex to regulate responses to jasmonate signaling. However, functional studies indicate that some aspects of jasmonate-mediated defense are not regulated by JA-Ile. Thus, it is not clear whether JA-Ile is best characterized as the master jasmonate regulator of defense, or if it regulates more specific aspects. We investigated possible functions of JA-Ile in anti-herbivore resistance of the wild tobacco , a model system for plant-herbivore interactions. We first analyzed the soluble and volatile secondary metabolomes of irxir, as, and WT plants, as well as an RNAi line targeting the jasmonate co-receptor CORONATINE INSENSITIVE 1 (ir), following a standardized herbivory treatment. irxir were the most similar to WT plants, having a ca. 60% overlap in differentially regulated metabolites with either asLOX3 or ir. In contrast, while at least 25 volatiles differed between ir or as and WT plants, there were few or no differences in herbivore-induced volatile emission between irxir and WT plants, in glasshouse- or field-collected samples. We then measured the susceptibility of jasmonate-deficient vs. JA-Ile-deficient plants in nature, in comparison to wild-type (WT) controls, and found that JA-Ile-deficient plants (irxir) are much better defended even than a mildly jasmonate-deficient line (as). The differences among lines could be attributed to differences in damage from specific herbivores, which appeared to prefer either one or the other jasmonate-deficient phenotype. We further investigated the elicitation of one herbivore-induced volatile known to be jasmonate-regulated and to mediate resistance to herbivores: ()-α-bergamotene. We found that JA was a more potent elicitor of ()-α-bergamotene emission than was JA-Ile, and when treated with JA, irxir plants emitted 20- to 40-fold as much ()-α-bergamotene than WT. We conclude that JA-Ile regulates specific aspects of herbivore resistance in . This specificity may allow plants flexibility in their responses to herbivores and in managing trade-offs between resistance, vs. growth and reproduction, over the course of ontogeny.
Mots clés
Manduca sexta, Nicotiana attenuata, direct and indirect defense, jasmonate signaling, jasmonoyl isoleucine (JA-Ile), plant metabolomics, plant-herbivore interactions
Référence
Front Plant Sci. 2018 06 14;9:787
