Fiche publication
Date publication
mars 2019
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SCHALLER Hubert
Tous les auteurs :
Forestier E, Romero-Segura C, Pateraki I, Centeno E, Compagnon V, Preiss M, Berna A, Boronat A, Bach TJ, Darnet S, Schaller H
Lien Pubmed
Résumé
Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays.
Mots clés
Biofuels, Enzyme Assays, Euphorbia, chemistry, Gene Expression Profiling, Intramolecular Transferases, genetics, Latex, chemistry, Plant Leaves, chemistry, Plant Proteins, genetics, Plants, Genetically Modified, Nicotiana, genetics, Triterpenes, metabolism
Référence
Sci Rep. 2019 03 18;9(1):4840
