Fiche publication
Date publication
février 2022
Journal
Science (New York, N.Y.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GAQUEREL Emmanuel
Tous les auteurs :
Bai Y, Yang C, Halitschke R, Paetz C, Kessler D, Burkard K, Gaquerel E, Baldwin IT, Li D
Lien Pubmed
Résumé
Although much is known about plant traits that function in nonhost resistance against pathogens, little is known about nonhost resistance against herbivores, despite its agricultural importance. leafhoppers, serious agricultural pests, identify host plants by eavesdropping on unknown outputs of jasmonate (JA)-mediated signaling. Forward- and reverse-genetics lines of a native tobacco plant were screened in native habitats with native herbivores using high-throughput genomic, transcriptomic, and metabolomic tools to reveal an -elicited JA-JAZi module. This module induces an uncharacterized caffeoylputrescine-green leaf volatile compound, catalyzed by a polyphenol oxidase in a Michael addition reaction, which we reconstitute in vitro; engineer in crop plants, where it requires a berberine bridge enzyme-like 2 (BBL2) for its synthesis; and show that it confers resistance to leafhoppers. Natural history-guided forward genetics reveals a conserved nonhost resistance mechanism useful for crop protection.
Mots clés
Animals, Biosynthetic Pathways, Catechol Oxidase, genetics, Crops, Agricultural, Cyclopentanes, metabolism, Genes, Plant, Hemiptera, Herbivory, Metabolome, Oxylipins, metabolism, Plant Leaves, metabolism, Synthetic Biology, Nicotiana, genetics, Transcriptome, Volatile Organic Compounds, chemistry
Référence
Science. 2022 02 4;375(6580):eabm2948
