Fiche publication
Date publication
mars 2026
Journal
Muscles (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GENY Bernard
Tous les auteurs :
Charles AL, Tetsi L, Quiring G, Barnig C, Giannini M, Meyer A, Lejay A, Lugnier C, Geny B
Lien Pubmed
Résumé
Peripheral arterial disease is a leading cause of amputation and/or death worldwide. Phosphodiesterase 4 (PDE 4) inhibitors demonstrated beneficial effects in ischemia-reperfusion (IR) settings, but whether PDE 4 inhibition protects skeletal muscle against IR deleterious effects is unknown. We therefore performed limb IR (two hours each) in twenty-one male Swiss mice (12-16-week-old) treated or not with Rolipram (1 mg/kg i.p. 30 min before ischemia and 5 min before reperfusion). The muscles were analyzed 4 h after the onset of ischemia. IR significantly increased leucocyte infiltration (93.13 ± 6.886 vs. 150.1 ± 18.38 cells/mg of muscle, < 0.05) and apoptosis (Bax/Bcl2 ratio, +239%, < 0.05), together with enhanced mitochondrial fission transcripts (+224% for Drp1, < 0.01 and +368%, < 0.0001 for Fis1), and decreased mitochondrial respiration and antioxidant defense. PDE 4 inhibition reduced leucocyte infiltration (150.1 ± 18.38 vs. 55.58 ± 13.83; < 0.01) and apoptosis (+67%, NS) in association with reduced fission markers (+91% for Drp 1 and +111%, < 0.05, for Fis 1). Muscle mitochondrial respiration did not improve. In conclusion, PDE 4 inhibition using Rolipram partly protected skeletal muscles against IR-induced deleterious effects. These data support further studies investigating the usefulness of leucocytes modulation in lower-limb IR and a potential beneficial effect of PDE 4 inhibition in peripheral arterial disease.
Mots clés
PDE 4, apoptosis, cyclic nucleotide phosphodiesterase (PDE) inhibition, fission, inflammation, ischemia–reperfusion, leucocytes, mitochondria, muscle, oxidative stress
Référence
Muscles. 2026 03 3;5(1):
