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Date publication
novembre 2025
Journal
Development (Cambridge, England)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr TAVIAN Manuela
Tous les auteurs :
Ivanovs A, Rybtsov S, Nnadi N, Fayon A, Gordon-Keylock S, Paruzina D, Anderson RA, Tavian M, Medvinsky A
Lien Pubmed
Résumé
During mammalian embryonic development, haematopoietic stem cells (HSCs) first emerge in the aorta-gonad-mesonephros (AGM) region. Human definitive HSCs emerge in low numbers and reside within the VE-CAD+CD45+ population consisting of 500-1000 cells. Accurate identification of the first HSCs emerging within this population is important for understanding their biology and underlying developmental mechanisms. Here, we characterised the expression of potential markers labelling HSCs during their emergence in the AGM at Carnegie stages (CS) 14-17. We found that the first definitive HSCs are marked by CD43, but not CD41, similar to the early haematopoietic progenitor cells differentiating in culture from human ES cells. We show that, during development in the AGM region and in the beginning of liver colonisation, HSCs remain GPI-80 negative, in contrast to liver HSCs at the later midtrimester foetal development. Together with our previous observations, these findings provide firm evidence that HSCs colonise human embryonic liver at CS17-18.
Mots clés
AGM region, Dorsal aorta, Haematopoietic stem cell, Human embryo
Référence
Development. 2025 11 6;:
