[Assembly of a Complex of an Immature 30S Ribosomal Subunit and the Era GTPase Maturation Factor from Staphylococcus aureus In Vitro for Structural Studies].

Date publication

janvier 2025

Journal

Molekuliarnaia biologiia

Auteurs

Membres identifiés du Cancéropôle Est :
Dr YUSUPOV Marat

Tous les auteurs :
Klochkova EA, Biktimirov AD, Bikmullin AG, Garaeva NS, Yusupov MM, Usachev KS

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/41090335

Résumé

Era GTPase of Staphylococcus aureus is a ribosome maturation factor. Era GTPase is involved in the late stages of small (30S) ribosome subunit assembly and regulates formation of the entire 70S ribosome. Study of the structure of the Era and 30S subunit complex from S. aureus will help in studying the processes of ribosome maturation and the mechanisms of protein synthesis regulation in this pathogenic microorganism. This paper presents protocols for obtaining Era GTPase and immature 30S ribosome subunits from S. aureus, as well as a protocol for assembling their complex for further structural studies.

Mots clés

30S ribosome subunit assembly, Era GTPase, Staphylococcus aureus, cryo-electron microscopy, ribosome, ribosome maturation factor

Référence

Mol Biol (Mosk). 2025 ;59(4):599-606