Single- vs. Multi-Walled Carbon Nanotubes: Differential Cellular Stress and Lipid Metabolism Effects in Macrophage Models.

Fiche publication

Date publication

septembre 2025

Journal

Nanomaterials (Basel, Switzerland)

Auteurs

Membres identifiés du Cancéropôle Est :
Pr RIHN Bertrand

Tous les auteurs :
Nahle S, Cassidy H, Matallanas D, Rihn BH, Joubert O, Ferrari L

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/41003036

Résumé

This study examines the toxicological effects of carbon nanotubes (CNTs) of different diameters-single-walled CNTs (SWCNT, 2 nm) and multi-walled CNTs (MWCNT, 74 nm)-on two macrophage cell lines, rat alveolar NR8383 cells and human differentiated THP-1. Using standardized exposure conditions and employing an integrated omics approach (transcriptomic and proteomic analyses), both CNT types were found to induce cellular stress responses and inflammation, especially in NR8383 cells, with notable involvement of the Sirtuin signaling pathway. After 24 h, MWCNTs uniquely disrupted lipid metabolism in NR8383 cells, resulting in foam cell formation and syncytia. While SWCNTs were less disruptive to metabolic pathways, they significantly altered gene regulation, particularly RNA splicing mechanisms. The dispersion medium-fetal bovine serum (FBS) versus human surfactant-also modulated the observed toxicological responses, highlighting the critical role of the protein corona in influencing CNT-cell interactions. These findings demonstrate that CNT diameter significantly affects cytotoxicity and cellular response pathways in a cell-type-specific manner.