Fiche publication
Date publication
septembre 2025
Journal
Comptes rendus biologies
Auteurs
Membres identifiés du Cancéropôle Est :
Dr YUSUPOV Marat
Tous les auteurs :
Nurulina L, Terossu S, Yusupov M
Lien Pubmed
Résumé
Ribosomes of highly evolved organisms are larger than their bacterial counterparts not only in terms of the number of proteins but also in terms of RNA length extensions. Some extensions do not fold into the three-dimensional structure of the ribosome in cryo-EM analysis and cannot be crystallized for X-ray examination. These expansion segments are not visible due to flexibility. In this mini-review, we propose an approach to study the chick embryo ribosome as an example of a highly evolved organism to visualize the expansion segments and study the mechanism of in situ tetramerization and crystallization of ribosomes.
Mots clés
Chick embryo, Cryo-EM, RNA extension segments, Ribosome, Structure
Référence
C R Biol. 2025 09 16;348:183-188
