Fiche publication
Date publication
janvier 2025
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JEANDIDIER Eric
Tous les auteurs :
M'kacher R, Colicchio B, Junker S, Schabat S, Belaiba R, Sontos L, Heidingsfelder L, Najar W, Plesch A, Voisin P, Dieterlen A, Jeandidier E, Carde P
Lien Pubmed
Résumé
The analysis of the origin of chromothripsis, catastrophic chromosomal rearrangements, has provided exceptional insights into various aspects of tumor progression and genetic disorders. Findings in chromothripsis have not only enhanced our understanding of genomic instability mechanisms, but also reshaped our views on chromosome mechanics. To date, the major mechanisms of chromothripsis described involve the incorporation of micronuclei into the primary nucleus and telomere crisis through the formation of dicentric chromosomes. Here, we reevaluated the impact of telomere and centromere sequences in the formation of micronuclei and anaphase bridges in cancer patients using our high throughput technique for the detection of telomere and centromere dysfunction and chromosomal instability biomarkers. We also discuss the emerging potential of exploiting telomere and centromere dysfunction combined with DNA damage as prognostic biomarkers and tools for personalized patient management.
Mots clés
Anaphase bridge, Centromere, Chromosomal instability, Chromothripsis, Micronuclei, Telomere
Référence
Methods Mol Biol. 2025 ;2968:441-455
