A F-ATCUN Peptide NMR Probe for Selective Cu or Ni Sensing in Complex Biological Media.

Fiche publication

Date publication

août 2025

Journal

Chemistry (Weinheim an der Bergstrasse, Germany)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr KIEFFER Bruno

Tous les auteurs :
Tilly MJ, Falcone E, Kieffer B, François YN, Faller P, Murr MD, Raibaut L

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/40827644

Résumé

We report the design and synthesis of ratiometric F-ATCUN (Xxx-Zzz-His) peptide probes for the selective and reversible detection of Ni⁺ and Cu⁺ by F NMR spectroscopy. In contrast to fluorescence-based approaches, F NMR enables background-free detection in biological media, due to the absence of fluorine in native biomolecules. The probes remain stable and functional in complex cell media (DMEM), enabling the detection of Cu⁺ and Ni⁺ by F NMR with a limit of detection (LOD) of 4 µM and quantification (LOQ) of 10 µM, relevant for studying copper-related pathologies.