Fiche publication
Date publication
juillet 2025
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SMEKTALA Frédéric
Tous les auteurs :
Zaiter R, Adamietz F, Désévédavy F, Strutynski C, Bailleul D, Smektala F, Buffeteau T, Vellutini L, Dussauze M
Lien Pubmed
Résumé
Bio-functionalized chalcogenide infrared optical glass fibers have been designed for evanescent wave mid-infrared spectroscopy. Surface biotinylation of the fiber tapered sensing zone has been achieved by reactivity of a maleimide function on sulfhydryl moieties of the glassy surface. Biotin-streptavidin interactions were studied by fiber evanescent wave spectroscopy. Kinetic measurement comparisons of functionalized and non-functionalized fiber surfaces for various protein concentrations have demonstrated the efficient bio-selectivity of the functionalized glass fibers. The protein enrichment of the functionalized glassy surface allows for a significant increase of the protein detection limit, greater than two orders of magnitude as compared to reference non-functionalized fibers. A detection of minute quantities at concentrations as low as 10 parts-per-billion is demonstrated. This study shows that bio-functionalized chalcogenide optical fibers allow to combine successfully surface bio-selectivity and infrared absorption fingerprints measurements to get a remarkable sensitivity enhancement of fiber evanescent wave detection methods in the mid infrared spectral range.
Référence
Sci Rep. 2025 07 1;15(1):21679
