First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period.

Fiche publication

Date publication

décembre 2020

Journal

Comparative biochemistry and physiology. Part D, Genomics & proteomics

Auteurs

Membres identifiés du Cancéropôle Est :
Dr HERGALANT Sébastien

Tous les auteurs :
Roche J, Hergalant S, Depp A, Ben Ammar I, Lafont AG, Policar T, Fontaine P, Milla S

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/32987329

Résumé

Dopamine (DA) is a ubiquitous neurotransmitter exerting a range of pleiotropic actions through two DA receptor families, the D1 and the D2. To date in vertebrates, a maximum of four receptor subtypes have been identified within the D1 family, D (former D), D (former D), D (former D and D) and D (former D), while the D2 family encloses five subtypes, D, D, D, D (former D or D) and D (former D or D). In teleosts, no study has investigated in parallel all the DA receptors to identify and localize the whole receptor repertoire from both families. In pikeperch, Sander lucioperca, a species of interest for aquaculture development, the existence, number and location of the DA receptors are totally unknown. To address these questions, RNA-seq with de novo transcriptome reconstruction, functional annotation and phylogenetic analysis were performed to characterize the transcript repertoire of DA receptors in the brain of female pikeperch at the pre-ovulatory period. Ten different cDNA were identified and showed to belong to the D1 family: two D, one D, one D and one D and to the D2 family: two spliced variants of D, one D, one D and one D Unlike zebrafish, the subtypes D and D have not yet been isolated in pikeperch. As expected D, D, D and D are mostly expressed in brain parts except for the cerebellum (D and D). The inter-species differences in the number of DA receptors and the inter-organ differences in the gene expression of all receptors support the complexity of the dopaminergic actions in vertebrate.