Crossborder generation of reproducible prostate and colon cancer organoids to study tumor progression and treatment resistance (CrossOids)

Fiche projet

Année

2025

Appel à projets

Appel à projets « Soutien à l'émergence de projets transfrontaliers » (Cancéropôle Est)

Acronyme

CrossOids

Resumé

Patient-derived organoids (PDO) is a powerful research tool for advancing drug discovery and personalized medicine, but there is still a long way to go to optimize reproducible large-scale assay. The current limitations of tumouroids are due to several factors, including the variability of the niche factors used, their stability and potential degradation and, of course, variations in handling by different researchers. The present consortium has to some extent demonstrated that mouse-derived tumouroids (MDT) mimic the human disease. In this project, we plan to link two platforms, the organoid research platform at KIT (https://www.healthtech.kit.edu/650.php) and GenomEast (https://www.igbmc.fr/en/plateforms-and-services/platforms/genomeast) at IGBMC, Strasbourg. We aim to standardize two types of MDT isolated from colon and prostate cancer using the automated cell culture unit of the organoid research platform of the KIT, which includes the CellXpress.ai™ system. The successful standardization of these organoids will be assessed by sequencing at the IGBMC platform at specific time points during the growth of the organoids. In addition, based on Team 1 and 2 expertise in the field of drug resistance, we will induce resistance to Standards Of Care (SOC) and screen using the ChemASAP platform (https://www.chemasap.kit.edu) from the KIT for novel drugs that can be used in the future in prediction studies for personalized medicine. The results gained could lead to new therapeutic strategies to overcome SOC-induced resistance.

The use of MDT will be a first step before moving on to standardizing the preparation of PDO. This common initiative will allow us to lay the groundwork for a larger initiative from the EUCOR region, where we want to join forces, not only in research but also in teaching. This will allow us to pave the way for future development where the whole preparation process could be automated and self-improved as in so-called self-driving labs.

Avec Dr Véronique ORIAN-ROUSSEAU (Karlsruhe Institute of Technology - KIT, Allemagne)