Pr DIDIER Pascal

Quantitative fluorescence imaging of the impact of the nucleocapsid domain deletion on the dynamics of HIV-1 Gag assembly.

Lysova I, Didier P, Mesaddeq N, Bouthenet J, Mely Y, Anton H

Methods Appl Fluoresc. 2026 03 19;:

Deciphering the Blinking Mechanisms of Dye-Loaded Organic Nanoparticles.

Biswas DS, Fanciullo G, Boglio B, Bordoni S, Rivalta I, Klymchenko AS, Didier P

J Phys Chem Lett. 2025 11 7;:11946-11954

Exploring excited-state proton transfer reactions in isothiazologuanosine, an isofunctional fluorescent analogue of guanosine.

Tkach O, Martinez-Fernandez L, Ruiz-Lera AE, Humbert N, Richert L, Dziuba D, Bombarda E, Bourderioux A, Hanser F, Didier P, Tor Y, Improta R, Léonard J, Mély Y

Phys Chem Chem Phys. 2025 09 3;:

Light-Harvesting Nanomaterials Based on Dyes for Energy Transfer and Amplified Biosensing.

Klymchenko AS, Biswas DS, Didier P

Adv Mater. 2025 08 5;:e01237

Lipophilic molecular rotor to assess the viscosity of oil core in nano-emulsion droplets.

Elhassan M, Faivre C, Anton H, Conzatti G, Didier P, Vandamme T, Elamin AS, Collot M, Anton N

Soft Matter. 2025 01 20;:

Targeted Photoactivatable Green-Emitting BODIPY Based on Directed Photooxidation-Induced Activation and its Application to Live Dynamic Super-Resolution Microscopy.

Saladin L, Le Berruyer V, Bonnevial M, Didier P, Collot M

Chemistry. 2024 10 4;:e202403409

Ferroelastic ionic organic crystals that self-heal to 95.

Al-Handawi MB, Commins P, Dalaq AS, Santos-Florez PA, Polavaram S, Didier P, Karothu DP, Zhu Q, Daqaq M, Li L, Naumov P

Nat Commun. 2024 09 16;15(1):8095

Red-Emitting Pyrrolyl Squaraine Molecular Rotor Reports Variations of Plasma Membrane and Vesicular Viscosity in Fluorescence Lifetime Imaging.

Pfister S, Lesieur J, Bourdoncle P, Elhassan M, Didier P, Anton N, Anton H, Collot M

Anal Chem. 2024 07 27;:

Piggybacking functionalized DNA nanostructures into live-cell nuclei.

Roozbahani GM, Colosi PL, Oravecz A, Sorokina EM, Pfeifer W, Shokri S, Wei Y, Didier P, DeLuca M, Arya G, Tora L, Lakadamyali M, Poirier MG, Castro CE

Sci Adv. 2024 07 5;10(27):eadn9423

Targeted Photoconvertible BODIPYs Based on Directed Photooxidation-Induced Conversion for Applications in Photoconversion and Live Super-Resolution Imaging.

Saladin L, Breton V, Le Berruyer V, Nazac P, Lequeu T, Didier P, Danglot L, Collot M

J Am Chem Soc. 2024 06 11;:

HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.

Tomishige N, Bin Nasim M, Murate M, Pollet B, Didier P, Godet J, Richert L, Sako Y, Mély Y, Kobayashi T

Nat Commun. 2023 11 21;14(1):7353

The human cellular protein NoL12 is a specific partner of the HIV-1 nucleocapsid protein NCp7.

Zgheib S, Taha N, Zeiger M, Glushonkov O, Lequeu T, Anton H, Didier P, Boutant E, Mély Y, Réal E

J Virol. 2023 09 11;:e0004023

Long-range Energy Transfer Between Dye-loaded Nanoparticles: Observation and Amplified Detection of Nucleic Acids.

Biswas DS, Gaki P, Da Silva EC, Combes A, Reisch A, Didier P, Klymchenko AS

Adv Mater. 2023 04 18;:e2301402

Tuning Directed Photooxidation-Induced Conversion of Pyrrole-Based Styryl Coumarin Dual-Color Photoconverters.

Saladin L, Dal Pra O, Klymchenko AS, Didier P, Collot M

Chemistry. 2023 03 15;:e202300685

Lysosomes targeting pH activable imaging-guided photodynamic agents.

Figliola C, Anton H, Sutter C, Chériaux C, Sutter A, Mazan V, Elhabiri M, Didier P, Jacquemin D, Ulrich G

Chembiochem. 2023 02 23;:e202300139

Tuning Directed Photooxidation Induced Conversion of Pyrrole-based Styryl Coumarins Dual-Color Photoconverters.

Saladin L, Dal Pra O, Klymchenko A, Didier P, Collot M

Chemistry. 2023 01 31;:

Dual-Color Photoconvertible Fluorescent Probes Based on Directed Photooxidation Induced Conversion for bioimaging.

Saladin L, Breton V, Dal Pra O, Klymchenko AS, Danglot L, Didier P, Collot M

Angew Chem Int Ed Engl. 2022 11 24;:

Upstream of N-Ras (Unr/CSDE1) Interacts with NCp7 and Gag, Modulating HIV-1 IRES-Mediated Translation Initiation.

Taha N, Zgheib S, Sharma KK, Humbert N, Boutant E, Didier P, Mély Y, Real E

Viruses. 2022 08 17;14(8):

Thienoguanosine, a unique non-perturbing reporter for investigating rotational dynamics of DNA duplexes and their complexes with proteins.

Grytsyk N, Richert L, Didier P, Dziuba D, Ciaco S, Mazzoleni V, Lequeu T, Mori M, Tor Y, Martinez-Fernandez L, Improta R, Mély Y

Int J Biol Macromol. 2022 07 31;213:210-225

Thienoguanosine brightness in DNA duplexes is governed by the localization of its ππ* excitation in the lowest energy absorption band.

Ciaco S, Gavvala K, Greiner V, Mazzoleni V, Didier P, Ruff M, Martinez-Fernandez L, Improta R, Mely Y

Methods Appl Fluoresc. 2022 Apr 26;:

Modelling quenching mechanisms of disordered molecular systems in the presence of molecular aggregates.

Fanciullo G, Conti I, Didier P, Klymchenko A, Léonard J, Garavelli M, Rivalta I

Phys Chem Chem Phys. 2022 Jan 5;:

Mucus-producing epithelial models for investigating the activity of gene delivery systems in the lung.

Sonntag T, Rapp M, Didier P, Lebeau L, Pons F, Casset A

Int J Pharm. 2021 Dec 24;:121423

Kinetics of protein-assisted nucleic acid interconversion monitored by transient time resolved fluorescence in microfluidic droplets.

Grytsyk N, Cianfarani D, Crégut O, Richert L, Boudier C, Humbert N, Didier P, Mély Y, Léonard J

Nucleic Acids Res. 2021 Aug 27;:

A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress.

Moeglin E, Desplancq D, Stoessel A, Massute C, Ranniger J, McEwen AG, Zeder-Lutz G, Oulad-Abdelghani M, Chiper M, Lafaye P, Di Ventura B, Didier P, Poterszman A, Weiss E

Cancers (Basel). 2021 Jul 1;13(13):

Fundamental photophysics of isomorphic and expanded fluorescent nucleoside analogues.

Dziuba D, Didier P, Ciaco S, Barth A, Seidel CAM, Mély Y

Chem Soc Rev. 2021 May 6;:

Density of surface charge is a more predictive factor of the toxicity of cationic carbon nanoparticles than zeta potential.

Weiss M, Fan J, Claudel M, Sonntag T, Didier P, Ronzani C, Lebeau L, Pons F

J Nanobiotechnology. 2021 Jan 6;19(1):5

The use of pore-forming toxins to image lipids and lipid domains.

Tomishige N, Murate M, Didier P, Richert L, Mély Y, Kobayashi T

Methods Enzymol. 2021 ;649:503-542

Gefitinib induces EGFR and α5β1 integrin co-endocytosis in glioblastoma cells.

Blandin AF, Cruz Da Silva E, Mercier MC, Glushonkov O, Didier P, Dedieu S, Schneider C, Devy J, Etienne-Selloum N, Dontenwill M, Choulier L, Lehmann M

Cell Mol Life Sci. 2020 Nov 5;:

pH-Dependent absorption spectrum of oxyluciferin analogues in the active site of firefly luciferase.

de Almeida Barbosa NM, Gosset P, Réal E, Ledentu V, Didier P, Ferré N

Phys Chem Chem Phys. 2020 10 7;22(38):21731-21740

What makes thienoguanosine an outstanding fluorescent DNA probe?

Kuchlyan J, Martinez-Fernandez L, Mori M, Gavvala K, Ciaco S, Boudier C, Richert L, Didier P, Tor Y, Improta R, Mély Y

J. Am. Chem. Soc.. 2020 Sep 11;:

Zinc Fingers in HIV-1 Gag Precursor Are Not Equivalent for gRNA Recruitment at the Plasma Membrane.

Boutant E, Bonzi J, Anton H, Nasim MB, Cathagne R, Réal E, Dujardin D, Carl P, Didier P, Paillart JC, Marquet R, Mély Y, de Rocquigny H, Bernacchi S

Biophys. J.. 2020 Jun 12;:

Excited-State Proton Transfer in Oxyluciferin and its Analogues.

Gosset P, Taupier G, Cregut O, Brazard J, Mély Y, Dorkenoo KD, Léonard J, Didier P

J Phys Chem Lett. 2020 Apr 20;:

Deciphering the pH-dependence of ground- and excited-state equilibria of thienoguanine.

Didier P, Kuchlyan J, Martinez-Fernandez L, Gosset P, Léonard J, Tor Y, Improta R, Mély Y

Phys Chem Chem Phys. 2020 Mar 25;:

Uniform Widespread Nuclear Phosphorylation of Histone H2AX Is an Indicator of Lethal DNA Replication Stress.

Moeglin E, Desplancq D, Conic S, Oulad-Abdelghani M, Stoessel A, Chiper M, Vigneron M, Didier P, Tora L, Weiss E

Cancers (Basel). 2019 Mar 13;11(3):

Optimized protocol for combined PALM-dSTORM imaging.

Glushonkov O, Réal E, Boutant E, Mély Y, Didier P

Sci Rep. 2018 Jun 8;8(1):8749

Encapsulation of Plant Viral Particles in Calcite Crystals.

Al-Handawi MB, Commins P, Shukla S, Didier P, Tanaka M, Raj G, Veliz FA, Pasricha R, Steinmetz NF, Naumov P

Adv Biosyst. 2018 05;2(5):e1700176

Simulation and Analysis of the Spectroscopic Properties of Oxyluciferin and Its Analogues in Water.

García-Iriepa C, Gosset P, Berraud-Pache R, Zemmouche M, Taupier G, Dorkenoo KD, Didier P, Léonard J, Ferré N, Navizet I

J Chem Theory Comput. 2018 04 10;14(4):2117-2126

Giant light-harvesting nanoantenna for single-molecule detection in ambient light.

Trofymchuk K, Reisch A, Didier P, Fras F, Gilliot P, Mely Y, Klymchenko AS

Nat Photonics. 2017 Oct;11(10):657-663

Coactivators and general transcription factors have two distinct dynamic populations dependent on transcription.

Vosnakis N, Koch M, Scheer E, Kessler P, Mély Y, Didier P, Tora L

EMBO J.. 2017 Jul;:

Oxyluciferin Derivatives: A Toolbox of Environment-Sensitive Fluorescence Probes for Molecular and Cellular Applications.

Ghose A, Maltsev OV, Humbert N, Hintermann L, Arntz Y, Naumov P, Mély Y, Didier P

J Phys Chem B. 2017 Feb;121(7):1566-1575

Cationic DOPC-Detergent Conjugates for Safe and Efficient in Vitro and in Vivo Nucleic Acid Delivery.

Pierrat P, Casset A, Didier P, Kereselidze D, Lux M, Pons F, Lebeau L

Chembiochem. 2016 Sep;17(18):1771-83

Targeting the replisome with transduced monoclonal antibodies triggers lethal DNA replication stress in cancer cells.

Desplancq D, Freund G, Conic S, Sibler AP, Didier P, Stoessel A, Oulad-Abdelghani M, Vigneron M, Wagner J, Mély Y, Chatton B, Tora L, Weiss E

Exp. Cell Res.. 2016 Mar;342(2):145-58

Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes.

Niko Y, Didier P, Mely Y, Konishi G, Klymchenko AS

Sci Rep. 2016 Jan;6:18870

Internalization mechanism of neuropeptide Y bound to its Y receptor investigated by high resolution microscopy.

Kempf N, Didier P, Postupalenko V, Bucher B, Mély Y

Methods Appl Fluoresc. 2015 May 6;3(2):025004

Efficient in vitro and in vivo pulmonary delivery of nucleic acid by carbon dot-based nanocarriers.

Pierrat P, Wang R, Kereselidze D, Lux M, Didier P, Kichler A, Pons F, Lebeau L

Biomaterials. 2015 May;51:290-302

Role of the nucleocapsid domain in HIV-1 Gag oligomerization and trafficking to the plasma membrane: a fluorescence lifetime imaging microscopy investigation.

El Meshri SE, Dujardin D, Godet J, Richert L, Boudier C, Darlix JL, Didier P, Mely Y, de Rocquigny H

J Mol Biol. 2015 Mar 27;427(6 Pt B):1480-94

The HIV-1 nucleocapsid protein recruits negatively charged lipids to ensure its optimal binding to lipid membranes.

Kempf N, Postupalenko V, Bora S, Didier P, Arntz Y, de Rocquigny H, Mely Y

J Virol. 2015 Feb;89(3):1756-67

Emission Properties of Oxyluciferin and Its Derivatives in Water: Revealing the Nature of the Emissive Species in Firefly Bioluminescence.

Ghose A, Rebarz M, Maltsev OV, Hintermann L, Ruckebusch C, Fron E, Hofkens J, Mely Y, Naumov P, Sliwa M, Didier P

J Phys Chem B. 2014 Nov 18.

Role of the nucleocapsid region in HIV-1 Gag assembly as investigated by quantitative fluorescence-based microscopy.

de Rocquigny H, El Meshri SE, Richert L, Didier P, Darlix JL, Mely Y

Virus Res. 2014 Nov 26;193:78-88

Generation of an intrabody-based reagent suitable for imaging endogenous proliferating cell nuclear antigen in living cancer cells.

Freund G, Desplancq D, Stoessel A, Weinsanto R, Sibler AP, Robin G, Martineau P, Didier P, Wagner J, Weiss E

J Mol Recognit. 2014 Sep;27(9):549-58

Collective fluorescence switching of counterion-assembled dyes in polymer nanoparticles.

Reisch A, Didier P, Richert L, Oncul S, Arntz Y, Mely Y, Klymchenko AS

Nat Commun. 2014 Jun 9;5:4089

The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells.

Rinaldi AS, Freund G, Desplancq D, Sibler AP, Baltzinger M, Rochel N, Mely Y, Didier P, Weiss E

Exp Cell Res. 2013 Apr 1;319(6):838-49

Identification of a thermally activated process in the Cy3 photobleaching mechanism.

Jouonang AL, Didier P, Mely Y

Phys Chem Chem Phys. 2012 Feb 7;14(5):1585-8

Characterization of the mechanisms of HIV-1 Vpr(52-96) internalization in cells.

Greiner VJ, Shvadchak V, Fritz J, Arntz Y, Didier P, Frisch B, Boudier C, Mely Y, de Rocquigny H

Biochimie. 2011 Oct;93(10):1647-58

A family of plasmodesmal proteins with receptor-like properties for plant viral movement proteins.

Amari K, Boutant E, Hofmann C, Schmitt-Keichinger C, Fernandez-Calvino L, Didier P, Lerich A, Mutterer J, Thomas CL, Heinlein M, Mély Y, Maule AJ, Ritzenthaler C

PLoS Pathog.. 2010 Sep;6(9):e1001119