Characterization of the active bacterial community involved in natural attenuation processes in arsenic-rich creek sediments.
Fiche publication
Date publication
mai 2011
Auteurs
Membres identifiés du Cancéropôle Est :
Mme SCHAEFFER-REISS Christine, Dr VAN DORSSELAER Alain
Tous les auteurs :
Bruneel O, Volant A, Gallien S, Chaumande B, Casiot C, Carapito C, Bardil A, Morin G, Brown GE Jr, Personne CJ, Le Paslier D, Schaeffer C, Van Dorsselaer A, Bertin PN, Elbaz-Poulichet F, Arsene-Ploetze F
Lien Pubmed
Résumé
Acid mine drainage of the Carnoules mine (France) is characterized by acid waters containing high concentrations of arsenic and iron. In the first 30 m along the Reigous, a small creek draining the site, more than 38% of the dissolved arsenic was removed by co-precipitation with Fe(III), in agreement with previous studies, which suggest a role of microbial activities in the co-precipitation of As(III) and As(V) with Fe(III) and sulfate. To investigate how this particular ecosystem functions, the bacterial community was characterized in water and sediments by 16S rRNA encoding gene library analysis. Based on the results obtained using a metaproteomic approach on sediments combined with high-sensitivity HPLC-chip spectrometry, several GroEL orthologs expressed by the community were characterized, and the active members of the prokaryotic community inhabiting the creek sediments were identified. Many of these bacteria are beta-proteobacteria such as Gallionella and Thiomonas, but gamma-proteobacteria such as Acidithiobacillus ferrooxidans and alpha-proteobacteria such as Acidiphilium, Actinobacteria, and Firmicutes were also detected.
Référence
Microb Ecol. 2011 May;61(4):793-810