What is the best technic to dislodge Staphylococcus epidermidis biofilm on medical implants?
Fiche publication
Date publication
août 2022
Journal
BMC microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr ZWETYENGA Narcisse
Tous les auteurs :
Moris V, Lam M, Amoureux L, Magallon A, Guilloteau A, Maldiney T, Zwetyenga N, Falentin-Daudre C, Neuwirth C
Lien Pubmed
Résumé
Bacterial biofilm can occur on all medical implanted devices and lead to infection and/or dysfunction of the device. In this study, artificial biofilm was formed on four different medical implants (silicone, piccline, peripheral venous catheter and endotracheal tube) of interest for our daily clinical and/or research practice. We investigated the best conventional technic to dislodge the biofilm on the implants and quantified the number of bacteria. Staphylococcus epidermidis previously isolated from a breast implant capsular contracture on a patient in the university hospital of Dijon was selected for its ability to produce biofilm on the implants. Different technics (sonication, Digest-EUR®, mechanized bead mill, combination of sonication plus Digest-EUR®) were tested and compared to detach the biofilm before quantifying viable bacteria by colony counting.
Mots clés
Biofilm quantification, Endotracheal tube, Enzymatic treatment, Medical implant, Peripheral venous catheter, Piccline, Silicone, Sonication, Staphylococcus epidermidis
Référence
BMC Microbiol. 2022 08 6;22(1):192