Size-Dependent Internalization Efficiency of Macrophages from Adsorbed Nanoparticle-Based Monolayers.
Fiche publication
Date publication
juillet 2021
Journal
Nanomaterials (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANSELME Karine, Dr PIEUCHOT Laurent
Tous les auteurs :
Petithory T, Pieuchot L, Josien L, Ponche A, Anselme K, Vonna L
Lien Pubmed
Résumé
Functional coatings based on the assembly of submicrometric or nanoparticles are found in many applications in the biomedical field. However, these nanoparticle-based coatings are particularly fragile since they could be exposed to cells that are able to internalize nanoparticles. Here, we studied the efficiency of RAW 264.7 murine macrophages to internalize physisorbed silica nanoparticles as a function of time and particle size. This cell internalization efficiency was evaluated from the damages induced by the cells in the nanoparticle-based monolayer on the basis of scanning electron microscopy and confocal laser scanning microscopy observations. The internalization efficiency in terms of the percentage of nanoparticles cleared from the substrate is characterized by two size-dependent regimes. Additionally, we highlighted that a delay before internalization occurs, which increases with decreasing adsorbed nanoparticle size. This internalization is characterized by a minimal threshold that corresponds to 35 nm nanoparticles that are not internalized during the 12-h incubation considered in this work.
Mots clés
endocytosis, internalization, macrophages, monolayers, nanoparticles, phagocytosis, physisorption
Référence
Nanomaterials (Basel). 2021 Jul 30;11(8):