Topographical curvature is sufficient to control epithelium elongation.
Fiche publication
Date publication
septembre 2020
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ANSELME Karine, Dr PIEUCHOT Laurent
Tous les auteurs :
Rougerie P, Pieuchot L, Dos Santos RS, Marteau J, Bigerelle M, Chauvy PF, Farina M, Anselme K
Lien Pubmed
Résumé
How biophysical cues can control tissue morphogenesis is a central question in biology and for the development of efficient tissue engineering strategies. Recent data suggest that specific topographies such as grooves and ridges can trigger anisotropic tissue growth. However, the specific contribution of biologically relevant topographical features such as cell-scale curvature is still unclear. Here we engineer a series of grooves and ridges model topographies exhibiting specific curvature at the ridge/groove junctions and monitored the growth of epithelial colonies on these surfaces. We observe a striking proportionality between the maximum convex curvature of the ridges and the elongation of the epithelium. This is accompanied by the anisotropic distribution of F-actin and nuclei with partial exclusion of both in convex regions as well as the curvature-dependent reorientation of pluricellular protrusions and mitotic spindles. This demonstrates that curvature itself is sufficient to trigger and modulate the oriented growth of epithelia through the formation of convex "topographical barriers" and establishes curvature as a powerful tuning parameter for tissue engineering and biomimetic biomaterial design.
Référence
Sci Rep. 2020 Sep 8;10(1):14784