Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles.
Fiche publication
Date publication
avril 2018
Journal
Bioconjugate chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHARBONNIERE Loïc
Tous les auteurs :
Cardoso Dos Santos M, Goetz J, Bartenlian H, Wong KL, Charbonnière LJ, Hildebrandt N
Lien Pubmed
Résumé
Fluorescent nanoparticles (NPs) have become irreplaceable tools for advanced cellular and subcellular imaging. While very bright NPs require excitation with UV or visible light, which can create strong autofluorescence of biological components, NIR-excitable NPs without autofluorescence issues exhibit much lower brightness. Here, we show the application of a new type of surface-photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. The combination of exceptionally high brightness, high photostability, and long photoluminecence (PL) lifetimes for highly efficient suppression of the short-lived autofluorescence allowed for time-gated PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low Tb-NP concentrations down to 12 pM. Detection of highly resolved long-lifetime (ms) PL decay curves from small (∼10 μm) areas within single cells within a few seconds emphasized the unprecedented photophysical properties of Tb-NPs for live-cell imaging that extend well beyond currently available nanometric imaging agents.
Mots clés
Cell Survival, Fluorescence, Fluorescent Dyes, chemistry, HeLa Cells, Humans, Microscopy, Fluorescence, methods, Nanoparticles, chemistry, Optical Imaging, methods, Terbium, chemistry
Référence
Bioconjug. Chem.. 2018 04 18;29(4):1327-1334