Molecular responses of alveolar epithelial A549 cells to chronic exposure to titanium dioxide nanoparticles: A proteomic view.
Fiche publication
Date publication
février 2016
Journal
Journal of proteomics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah, Dr VAN DORSSELAER Alain
Tous les auteurs :
Armand L, Biola-Clier M, Bobyk L, Collin-Faure V, Diemer H, Strub JM, Cianferani S, Van Dorsselaer A, Herlin-Boime N, Rabilloud T, Carriere M
Lien Pubmed
Résumé
Although the biological effects of titanium dioxide nanoparticles (TiO2-NPs) have been studied for more than two decades, the mechanisms governing their toxicity are still unclear. We applied 2D-gel proteomics analysis on A549 epithelial alveolar cells chronically exposed for 2months to 2.5 or 50μg/mL of deeply characterized TiO2-NPs, in order to obtain comprehensive molecular responses that may reflect functional outcomes. We show that exposure to TiO2-NPs impacts the abundance of 30 protein species, corresponding to 22 gene products. These proteins are involved in glucose metabolism, trafficking, gene expression, mitochondrial function, proteasome activity and DNA damage response. Besides, our results suggest that p53 pathway is activated, slowing down cell cycle progression and reducing cell proliferation rate. Moreover, we report increased content of chaperones-related proteins, which suggests homeostasis re-establishment. Finally, our results highlight that chronic exposure to TiO2-NPs affects the same cellular functions as acute exposure to TiO2-NPs, although lower exposure concentrations and longer exposure times induce more intense cellular response.
Mots clés
Cell Line, Tumor, Epithelial Cells, metabolism, Humans, Nanoparticles, Proteome, metabolism, Proteomics, Pulmonary Alveoli, metabolism, Respiratory Mucosa, metabolism, Titanium, chemistry
Référence
J Proteomics. 2016 Feb;134:163-73