Increased Levels of Interleukin-17A Exosomes in Psoriasis.
Fiche publication
Date publication
août 2019
Journal
Acta dermato-venereologica
Auteurs
Membres identifiés du Cancéropôle Est :
Pr ADOTEVI Olivier, Pr AUBIN François, Dr GARRIDO Carmen, Dr GOBBO Jessica, Dr NARDIN Charlée
Tous les auteurs :
Jacquin-Porretaz C, Cordonnier M, Nardin C, Boullerot L, Chanteloup G, Vautrot V, Adotevi O, Garrido C, Gobbo J, Aubin F
Lien Pubmed
Résumé
Exosomes are involved in modulating the immune system and mediating communication between cells. The aim of this study was to investigate the involvement of exosomes in psoriasis. Exosomes from patients were analysed by nanoparticle tracking analysis and protein expression was analysed by western blotting. The concentration of HSP70 was determined by an enzyme-linked immunosorbent assay, and concentrations of interleukin (IL)-1β, IL-2, IL-6, IL-10, IL-17A and tumour necrosis factor alpha (TNF-α) were determined by flow cytometry. Based on the severity of psoriasis, evaluated by body surface area (≤ 10% vs. > 10%), 2 groups of patients were compared (49 with mild psoriasis and 71 with moderate-to-severe psoriasis). The number (2.52×1011 ± 2.29×1010 vs. 1.79×1011 ± 1.93×1010, p = 0.19) and size (94.44±22.00 nm vs. 96.87 ± 28.30 nm, p = 0.72) of exosomes were not significantly different in the 2 groups of patients as HSP70 in the exosomes. IL-17A exosome levels were significantly higher in patients with moderate-to-severe psoriasis compared with those with mild psoriasis (p = 0.02). There were no significant differences in levels of TNF-α, IL-1, IL-2, IL-6 and IL-10. This study shows, for the first time, the presence of circulating exosomes in patients with psoriasis. These data confirm the involvement of circulating exosomes in psoriasis, in particular in moderate-to-severe psoriasis, through IL-17A-producing exosomes.
Mots clés
IL-17, psoriasis, exosomes
Référence
Acta Derm. Venereol.. 2019 08 22;: