RNA sequencing and transcriptome arrays analyses show opposing results for alternative splicing in patient derived samples.
Fiche publication
Date publication
juin 2017
Journal
BMC genomics
Auteurs
Lien Pubmed
Résumé
RNA sequencing (RNA-seq) and microarrays are two transcriptomics techniques aimed at the quantification of transcribed genes and their isoforms. Here we compare the latest Affymetrix HTA 2.0 microarray with Illumina 2000 RNA-seq for the analysis of patient samples - normal lung epithelium tissue and squamous cell carcinoma lung tumours. Protein coding mRNAs and long non-coding RNAs (lncRNAs) were included in the study.
Mots clés
Differential exon usage, Differential expression analysis, Microarrays, RNA sequencing, Splicing
Référence
BMC Genomics. 2017 Jun;18(1):443