Sperm imprinting integrity in seminoma patients?
Fiche publication
Date publication
octobre 2018
Journal
Clinical epigenetics
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BINQUET Christine, Pr FAUQUE Patricia
Tous les auteurs :
Bruno C, Blagoskonov O, Barberet J, Guilleman M, Daniel S, Tournier B, Binquet C, Choux C, Fauque P
Lien Pubmed
Résumé
Testicular germ cell tumor such as seminoma is strongly associated with male reproductive problems commonly associated with the alteration of sperm parameters as described in testicular dysgenesis syndrome. Interestingly, numerous studies have reported that the precursor of germ cell cancer, germ cell neoplasia in situ (GCNIS), present similarities to fetal gonocytes, specifically characterized by global DNA hypomethylation particularly on imprinting sequences. These disorders may have a common origin derived from perturbations of embryonal programming during fetal development. Presently, there is no available information concerning the sperm DNA methylation patterns of testicular cancer patients. For the first time, we evaluated the sperm imprinting of seminoma patients. A total of 92 cryopreserved sperm samples were included, 31 before seminoma treatment (S): 23 normozoospermic (SN) and 8 oligozoospermic (SO) and 61 sperm controls samples: 31 normozoospermic (N) and 30 oligozoospermic (O). DNA methylation levels of seven differentially methylated regions (DMRs) of imprinted genes [H19/IGF2: IG-DMR (CTCF3 and CTCF6 of H19 gene); IGF2-DMRs (DMR0 and DMR2); MEG3/DLK1:IG-DMR; SNURF:TSS-DMR; KCNQ1OT1:TSS-DMR] were assessed by pyrosequencing. All comparative analyses were adjusted for age.
Mots clés
Imprinted genes, Oligozoospermia, Seminoma, Sperm DNA methylation, Testicular dysgenesis syndrome (TDS), Testicular germ cell tumor (TGCT)
Référence
Clin Epigenetics. 2018 Oct 19;10(1):125