Effect of different agents onto multidrug resistant cells revealed by fluorescence correlation spectroscopy.
Fiche publication
Date publication
janvier 2007
Auteurs
Lien Pubmed
Résumé
Fluorescence correlation spectroscopy (FCS), which is a sensitive and non invasive technique, has been used to characterize the plasma membrane fluidity and heterogeneity of multidrug resistant living cells. At the single cell level, the effects of different membrane agents present in the extra-cellular medium have been analyzed. Firstly, we reveal a modi. cation of plasma membrane microviscosity according to the addition of a fluidity modulator, benzyl alcohol. In the other hand, revertant such as verapamil and cyclosporin-A appears to act more specifically on the slow diffusion sites as microdomains.
Référence
Ann Phys-paris. 2007;32(2-3):139-41.