Osteoblast cell activity on calcium phosphate layers grown on glass by a laser-liquid-solid interaction.
Fiche publication
Date publication
janvier 2007
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LAQUERRIERE Patrice
Tous les auteurs :
Dimitrova M, Pramatarova L, Pecheva E, Laquerriere P, Montgomery P, Petrova A, Altankov G
Lien Pubmed
Résumé
A calcium phosphate layer was grown on glass by a laser-liquid-solid interaction (LLSI) process in simulated body fluid (SBF). Glass samples with a layer grown by simple soaking in the SBF (i.e. without laser irradiation) were prepared for comparison. Formation of an inhomogeneous calcium phosphate (CaP) layer on both laser-treated and non-treated samples was observed. The results showed that the laser irradiation did not change the layer structure and morphology but yielded the growth of a thicker CaP layer. With increasing load the elasticity and the hardness increased for both laser-treated and non-treated samples. Furthermore, we tested the osteoblast cell activity of the CaP layers grown on the laser-treated and non-treated samples. Toxicity test showed that the viability of the cells on the layer grown by the LLSI process was over 95%. A permanent increase in the cell number was observed for both groups of samples, and it was more stable on the laser-treated surfaces. The latter showed a higher cell number after 7 days of cell culturing. A slower increase, resulting in a lower cell numbers was observed for the samples untreated with laser irradiation.
Référence
. 2007 Jan;9(1):240-3.