Poly(L-lysine) nanostructured particles for gene delivery and hormone stimulation.
Fiche publication
Date publication
mars 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BENKIRANE-JESSEL Nadia, Pr MAINARD Didier, Dr VOEGEL Jean-Claude
Tous les auteurs :
Zhang X, Oulad-Abdelghani M, Zelkin AN, Wang Y, Haikel Y, Mainard D, Voegel JC, Caruso F, Benkirane-Jessel N
Lien Pubmed
Résumé
In this work, we designed replica particles based on poly (L-lysine) (PLL) polymers crosslinked via a homobifunctional linker to support coadsorption of a plasmid DNA and a peptide hormone for concurrent transfection and induction of a cellular function. PLL replica particles (PLL(RP)) were prepared by infiltrating polymer into mesoporous silica (MS) particles, crosslinking the adsorbed chains by using a homobifunctional crosslinker and finally removing the template particles. Moreover, we verified their cytotoxicity. Furthermore, based on this PLL(RP) gene delivery system, we simultaneously evaluated the melanin stimulation and gene expression in these cells by fluorescence microscopy. To further understand the bi-functionality, we labeled the SPT7pTL and PGA-alpha-MSH with YOYO-1 and Rhodamine, respectively, to follow its intracellular pathway by confocal microscopy. Our data suggests that the PLL(RP) is a promising vector for gene therapy and hormone stimulation.
Référence
Biomaterials. 2010 Mar;31(7):1699-706