Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis.

Fiche publication


Date publication

mars 2010

Auteurs

Membres identifiés du Cancéropôle Est :
Mme SCHAEFFER-REISS Christine, Dr VAN DORSSELAER Alain


Tous les auteurs :
Castro AJ, Bednarczyk A, Schaeffer-Reiss C, Rodriguez-Garcia MI, Van Dorsselaer A, de Dios Alche J

Résumé

In the present paper, we have used 2-DE coupled to MS analysis to examine the molecular variability of the Ole e 1 allergen in three olive cultivars (cvs). Our results confirmed that the predicted polymorphism of Ole e 1 at cDNA level is extended to the expressed protein. The profiles of both the Ole e 1 peptides and the N-glycan variants significantly changed among cvs. We observed that Picual and Arbequina cvs presented the highest and lowest degree of Ole e 1 polymorphism, respectively. Some of these peptides and N-glycans were distributed in a cv-specific manner. The putative implications of this molecular polymorphism in the development of the allergy symptoms are discussed.

Référence

Proteomics. 2010 Mar;10(5):953-62.